Huiwang AiAssistant Professor of ChemistryChemistry ahuiwang@ucr.edu(951) 827-3710
CAREER: Expanding the Toolbox of Encodable Fluorescent Probes to Image Macrophage Redox Dynamics
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AWARD NUMBER
006622-005
FUND NUMBER
21230
STATUS
Closed
AWARD TYPE
3-Grant
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AWARD EXECUTION DATE
4/12/2015
BEGIN DATE
5/1/2014
END DATE
4/30/2019
AWARD AMOUNT
$2,761
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Sponsor Information
SPONSOR AWARD NUMBER
SPONSOR
SPONSOR TYPE
FUNCTION
Organized Research
PROGRAM NAME
Proposal Information
PROPOSAL NUMBER
14010051
PROPOSAL TYPE
New
ACTIVITY TYPE
Basic Research
PI Information
PI
Ai, Huiwang
PI TITLE
Other
PI DEPTARTMENT
Chemistry
PI COLLEGE/SCHOOL
College of Nat & Agr Sciences
CO PIs
Project Information
ABSTRACT
With this award from the Chemistry of Life Processes Program in the Chemistry Division, Dr. Huiwang Ai and his research team at the University of California, Riverside are developing new methods for the detection of hydrogen sulfide and other redox-active molecules in living cells. Fluorescent proteins in nonnative topologies with nonnatural chromophore structures will be exploited as novel redox probes. These selective fluorescent probes will be utilized to uncover the mechanisms of redox response and regulation in macrophage immune cells. The project will result in a general strategy for probing redox-active small signaling molecules in subcellular locations and in space close to proteins of interest.
It is widely accepted by the scientific community that cells use redox-active molecules to generate and relay signals. The misregulation of these processes is linked to a large number of diseases. Dr. Ai and his coworkers have discovered the means to selectively observe redox-active molecules in live cells. This project will be a necessary step toward a more comprehensive understanding of redox signaling and regulation. The project will also train graduate and undergraduate students for specialized expertise at the chemistry-biology interface. Middle and high school students from the local STEM school will be supported for research experiences. Curriculum enhancements and outreach activities will be carried out to further broaden the impacts of this project.(Abstract from NSF)
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